Goat anti-Rabbit IgG (H+L) Poly-HRP, HRP 32260 from Thermo Fisher Scientific

Product Specs
ItemGoat anti-Rabbit IgG (H+L) Poly-HRP, HRP
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number32260
Quantity500 µL
ApplicationsELISA, Immunohistochemistry, Western Blot
Conjugate/TagHRP
FormatPBS with proprietary stabilizer and no preservative
ReactivityRabbit
TargetRabbit IgG (H+L) Poly-HRP
IsotypeIgG
HostGoat
Antibody TypeSecondary
ClonalityPolyclonal
Concentration0.5 mg/mL
Storage Temperature4° C
PurityAntigen affinity chromatography
ImmunogenPurified Rabbit IgG, whole molecule
Storage4° C

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Cancer-associated fibroblasts actively compress cancer cells and modulate mechanotransductionNature CommunicationsNovember 1, 2023Jorge Barbazan, Carlos Pérez-González, Manuel Gómez-González, Mathieu Dedenon, Sophie Richon, Ernest Latorre, Marco Serra, Pascale Mariani, Stéphanie Descroix, Pierre Sens, et al.antibody,–,1–200,1–200,1–400,–,A11011,Thermo Fisher Scientific Anti-rabbit HRP,Goat anti-Rabbit polyclonal antibody,,,,,,32260,Thermo Fisher Scientific ,Against Fibronectin,–,–,–,–,"1–10,000",, ,Against MyosinIIA,–,–,–,–,1–5000,, ,Against

(2) Isolation and Characterization of Cetacean Cell-Derived Extracellular VesiclesAnimals (Basel)October 24, 2023Valentina Moccia, Cinzia Centelleghe, Ilaria Giusti, Antonella Peruffo, Vincenza Dolo, Sandro Mazzariol, Valentina ZappulliThen, membranes were incubated with a peroxidase-conjugated secondary antibody (1:3000; anti-Rabbit #32260 or anti-Mouse #32230, Thermo Fisher Scientific) diluted in TBS-T for 1 h at room temperature. Reactive

(3) Phospholipid isotope tracing reveals β-catenin-driven suppression of phosphatidylcholine metabolism in hepatocellular carcinomabioRxivOctober 21, 2023Chad VanSant-Webb, Hayden K. Low, Junko Kuramoto, Claire E. Stanley, Hantao Qiang, Audrey Su, Alexis N. Ross, Chad G. Cooper, James E. Cox, Scott A. Summers, et al.washed with TBS-T and incubated with 1:10000 goat anti-rabbit poly-HRP secondary antibody (Thermo, 32260) and 1:10,000 anti-β-actin-peroxidase antibody (Sigma, A3854) in 5% bovine serum album/TBS-T for 1 hr.

(4) A cyclin-dependent kinase-mediated phosphorylation switch of disordered protein condensationNature CommunicationsOctober 9, 2023Juan Manuel Valverde, Geronimo Dubra, Michael Phillips, Austin Haider, Carlos Elena-Real, Aurélie Fournet, Emile Alghoul, Dhanvantri Chahar, Nuria Andrés-Sanchez, Matteo Paloni, et al.goat anti-rabbit (Invitrogen, A11011; IF, 1:1000) Goat anti-rabbit IgG (H + L) HRP (Thermo Fisher, # 32260; WB, 1:10,000). Mapping of Ki-67-CR phosphorylation sites by mass spectrometry 6.5 µg of GFP-Ki-67-CR

(5) Long Noncoding RNA TALAM1 Is a Transcriptional Target of the RUNX2 Transcription Factor in Lung AdenocarcinomaCurrent issues in molecular biologyAugust 24, 2023Bermúdez G, Bernal C, Otalora A, Sanchez P, Nardocci G, Cañas A, Lopez-Kleine L, Montecino M, Rojas A.The membrane was washed and incubated with goat anti-rabbit IgG Poly-HRP (Thermo Fisher Scientific, 32260) at a 1:1000 dilution as the secondary antibody. 2.8. Statistical Analysis To identify statistically

(6) Regulation of store-operated Ca2+ entry by IP3 receptors independent of their ability to release Ca2+eLifeJuly 19, 2023Pragnya Chakraborty, Bipan Kumar Deb, Vikas Arige, Thasneem Musthafa, Sundeep Malik, David I Yule, Colin W Taylor, Gaiti Hasan(1:3000 anti-mouse, Cell Signaling Technology Cat# 7076 S; or 1:5000 anti-rabbit, ThermoScientific Cat# 32260). After 3 washes, HRP was detected using Pierce ECL Western Blotting Substrate (ThermoFisher, Cat# 32106)

(7) Decellularized Lung Extracellular Matrix Scaffold Promotes Human Embryonic Stem Cell Differentiation towards Alveolar ProgenitorsCell JJune 28, 2023Afshin Noori, Mohammad Reza Mokhber Dezfouli, Sareh Rajabi, Fatemeh Ganji, Zahra Ghezelayagh, Elie El Agha, Hossein Baharvand, Sirous Sadeghian Chaleshtori, Yaser TahamtaniAbcam, USA). On the following day, samples were stained with HRPconjugated secondary antibody (1:1000; 32260, Thermo Fisher Scientific, Germany) The rinsed sections were incubated with diaminobenzidine for 20

(8) Deficiency of germinal center kinase TRAF2 and NCK-interacting kinase (TNIK) in B cells does not affect atherosclerosisFrontiers in Cardiovascular MedicineMay 5, 2023Bram W. van Os, Pascal J. H. Kusters, Myrthe den Toom, Linda Beckers, Claudia M. van Tiel, Winnie G. Vos, Elize de Jong, Arnd Kieser, Cindy van Roomen, Christoph J. Binder, et al.control. TNIK and NF-κB p65 were visualized by staining with HRP conjugated Goat anti-Rabbit (Invitrogen, #32260) and Goat anti-Mouse (Invitrogen, #32330). Phospho-NF-κB p65 and alpha-tubulin were staining with IRDye

(9) Aberrant somatic calcium channel function in cNurr1 and LRRK2-G2019S micenpj Parkinson's DiseaseApril 7, 2023Olga Skiteva, Ning Yao, Ioannis Mantas, Xiaoqun Zhang, Thomas Perlmann, Per Svenningsson, Karima Cherguisecondary horseradish peroxidase-linked Anti-Rabbit IgG (H + L) (Thermo Scientific, Rockford, USA, Cat. No. 32260, 1:5000 dilution) or Anti-Mouse IgG (H + L) (Thermo Scientific, Cat. No. 32230, Rockford, US, 1:5000

(10) Intrafusal-fiber LRP4 for muscle spindle formation and maintenance in adult and aged animalsNature communicationsFebruary 10, 2023Cao R, Chen P, Wang H, Jing H, Zhang H, Xing G, Luo B, Pan J, Yu Z, Xiong WC, et al.from the Jackson ImmunoResearch. Horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Cat# 32260) and goat anti-mouse IgG antibodies (Cat# 32230) were from Thermo Fisher Scientific and used at 1:4000

(11) Multidrug resistance transporter-1 dysfunction perturbs meiosis and Ca2+ homeostasis in oocytesReproductionJanuary 1, 2023Dalileh Nabi, Davide Bosi, Neha Gupta, Nidhi Thaker, Rafael Fissore, Lynae M Brayboy#AM4300, Applied Biosystem), with 1:1000 dilution in 5% milk/TBS-T. Anti-rabbit IgG (H+L) poly-HRP (Cat. #32260, Invitrogen), with 1:10,000 dilution in TBS-T and anti-mouse IgG HRP conjugated (Cat. #DC02L, Calbiochem),

(12) Transmembrane protein 108 inhibits the proliferation and myelination of oligodendrocyte lineage cells in the corpus callosumMolecular BrainDecember 1, 2022Yongqiang Wu, Yanzi Zhong, Xufeng Liao, Xiangguang Miao, Jianbo Yu, Xinsheng Lai, Yu Zhang, Chaolin Ma, Haili Pan, Shunqi Wangblotting) was kindly presented by Dr. J. Liu [20]; Goat anti-rabbit IgG poly-HRP secondary antibody (32260) and Goat anti-rat IgG poly-HRP secondary antibody (31471) were purchased from Thermo Fisher Scientific

(13) L-Citrulline Supplementation Reduces Blood Pressure and Myocardial Infarct Size under Chronic Intermittent Hypoxia, a Major Feature of Sleep Apnea SyndromeAntioxidants (Basel)November 24, 2022Bilgehan Ozcan, Britanny Blachot-Minassian, Stéphanie Paradis, Lucile Mazière, Marie Chambion-Diaz, Sophie Bouyon, Jean-Louis Pépin, Vincent Pialoux, Claire Arnaud, Christophe Moinard, et al.were incubated with goat anti-rabbit poly-horseradish peroxidase (HRP) (1:2000 antibody Invitrogen 32260 mixed with HRP conjugate) for 1 h and washed again. Added to the wells was 100 µL of 3,3′,5′-Tétraméthylbenzidine

(14) Exploring Amodiaquine's Repurposing Potential in Breast Cancer Treatment-Assessment of In-Vitro Efficacy & Mechanism of ActionInternational journal of molecular sciencesSeptember 28, 2022Parvathaneni V, Chilamakuri R, Kulkarni NS, Baig NF, Agarwal S, Gupta V.HRP-conjugated antibodies: goat anti-mouse (#31430) poly-HRP (1:10,000 dilution) and goat anti-rabbit (#32260) (1:10,000 dilution) (Thermo Fisher Scientific, Waltham, MA, USA) for 1 h at room temperature and subjected

(15) Transcriptional regulation of CDKN2A/p16 by sirtuin 7 in senescenceMolecular Medicine ReportsSeptember 27, 2022Sergio RodrÃguez, Litzy Gisella BermÃºdez, Daniel GonzÃ¡lez, Camila Bernal, Alejandra CaÃ±as, Teresa Moralesâ€‘RuÃz, Berta HenrÃquez, Adriana Rojascontrol. Goat anti-Rabbit IgG Poly-HRP was used as secondary antibody, dilution 1/5000, incubation 2 h (32260 Thermo Fisher Scientific), at room temperature. The immunoblots were visualized in CL-Xposure Film using

(16) Modulatory Role of TPPP3 in Microtubule Organization and Its Impact on Alpha-Synuclein PathologyCellsSeptember 27, 2022Judit OlÃ¡h, Attila Lehotzky, Tibor SzÃ©nÃ¡si, TÃmea Berki, Judit OvÃ¡diGermany)",A2554,1:2000 cELISA; 1:5000 other "Anti-rabbit IgG, HRP-linked","Thermo Fisher Scientific (Waltham, MA, USA)",32260,1:2000 cELISA "Anti-mouse IgG, Alexa-546 linked","Thermo Fisher Scientific (Waltham, MA, USA)",A11003,1:1000 Antibodies

(17) The absence of the queuosine tRNA modification leads to pleiotropic phenotypes revealing perturbations of metal and oxidative stress homeostasis in Escherichia coli K12MetallomicsSeptember 24, 2022Leticia Pollo-Oliveira et al.with 1:5000 goat anti-rabbit IgG (H + L) Poly-HRP Secondary Antibody, HRP (Thermo Fisher Scientific, # 32260) and revealed using Clarity Western ECL Substrate (Bio-Rad, #1705061). The anti-FNR antibody and the

(18) 2'-O-Methylation of the second transcribed nucleotide within the mRNA 5' cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasionNucleic acids researchSeptember 9, 2022Drazkowska K, Tomecki R, Warminski M, Baran N, Cysewski D, Depaix A, Kasprzyk R, Kowalska J, Jemielity J, Sikorski PJ.membranes were incubated with secondary horseradish peroxidase (HRP)-conjugated goat anti-rabbit antibody (32260, Thermo Fisher Scientific) diluted 10 000-fold in PBST for 1 h at room temperature. Detection was performed

(19) Suppression of FAM83D Inhibits Glioma Proliferation, Invasion and Migration by Regulating the AKT/mTOR Signaling PathwayTranslational OncologyAugust 1, 2022Xia Li, Cui Sun, Jing Chen, Ji-Fen Ma, Yi-Heng Panincubation with goat polyclonal to rabbit horseradish peroxidase (HRP)-labeled secondary antibodies (32260, 1:10000, Thermo Fisher Scientific Inc., USA) at 37°C for 2 h, which was also terminated by three washes

(20) Gene therapy restores mitochondrial function and protects retinal ganglion cells in optic neuropathy induced by a mito-targeted mutant ND1 geneGene TherapyJune 1, 2022Yuan Liu, Jeremy D. Eastwood, Diego E. Alba, Sindhu Velmurugan, Ning Sun, Vittorio Porciatti, Richard K. Lee, William W. Hauswirth, John Guy, Hong YuSecondary probing with anti-mouse (32230, ThermoFisher, 1:5000) or anti-rabbit HRP-conjugated antibodies (32260, ThermoFisher, 1:5000) was performed for 1 h, followed by detection using ECL reagents (ThermoFisher)

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Goat anti-Rabbit IgG (H+L) Poly-HRP, HRP

Goat anti-Rabbit IgG (H+L) Poly-HRP, HRP from Thermo Fisher Scientific

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Goat anti-Rabbit IgG (H+L) Poly-HRP, HRP from Thermo Fisher Scientific

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